genus Pinus taeda , or more commonly the loblolly true pine , is quite an challenging being . It ’s found in Southeastern United States and is commercially grown in extensive plantations due to its gamey time value in the lumber trade . A novel genetic subject area has revealed that it has a genome even bigger than that of world - indeed , turgid than any other genome examined so far .
Traditional deoxyribonucleic acid sequencing involves a technique evolve by Frederick Sanger in 1977 call , not amazingly , Sanger sequencing . Although this has proved implausibly utilitarian over the years , it can only be used on modest DNA sequences of around 100 to 1000 base pairs , which are the building blocks of DNA . To put that into perspective , the human genome has over3 billionof those . sequence of multicellular organisms has demonstrated that the size of the genome does not inevitably prescribe the complexity of the being .
But there is a result to the limits levy by Sanger sequencing;shotgun sequence . This is where large strands of DNA are dampen up into random , smaller and more easy to handle fragment . These fragment can then be sequenced using Sanger sequencing , which is also called chain - termination . After several repeat rounds of this atomisation and sequencing , a computer will name overlapping sequences and employ this to run up together a single continuous sequence . But the loblolly pine ’s sequence was so unwieldy due to the act of insistent sequences that programs scramble to place properly , that even this method could n’t quite cut it . So what the scientists did was modify the shotgun approach by first generating clones which they organise , mapped and then sequenced .
The results were published in the journalGenome Biology , and it was found that the loblolly pine genome contains22.18 billion theme brace ; that is more than seven time the number found in the human genome . 82 % of this was , however , found to be insistent sequence , in comparison to only 25 % in the human genome . Not only that , but they identified numerous genes with important single-valued function , such as disease resistance , which could be important in the lumber industry .
This novel and in force approach can now be apply to sequence other complex organism , increase our cognition base even more .